- It is good practice to check image accessibility for color blind people before publication.
- A simple ImageJ script can simulate how images appear to different types of color blindness.
- Red/green overlays are problematic for protanopia and deuteranopia; showing separate grayscale channels is a recommended solution.
- Providing separated channels alongside overlays improves accessibility without changing figure layout.
- The script and approach can be freely used and feedback from color blind individuals is welcomed.