Host ESCRT machinery orchestrates the assembly of tomato spotted wilt virus ribonucleoproteins - PubMed
7 hours ago
- #RNA replication
- #ESCRT machinery
- #Tomato spotted wilt virus
- Host ESCRT machinery is involved in the assembly of tomato spotted wilt virus (TSWV) ribonucleoproteins (RNPs).
- Negative-strand RNA viruses encapsidate their genomic RNA with nucleocapsid proteins, forming RNPs to protect against degradation.
- TSWV nucleocapsid (N) and RNA polymerase (L) proteins colocalize at the trans-Golgi network (TGN) in a replicon-RNA-dependent manner.
- ESCRT mutants (snf7Δ, bro1Δ, doa4Δ) impair N localization to TGN and RNP formation, while vps36Δ affects RNA replication but not N localization.
- ESCRT components (SNF7, BRO1, DOA4) are recruited to TGN by TSWV N or L proteins, indicating additional roles in RNA replication beyond N transport.
- ESCRT machinery ensures accurate and efficient RNP assembly by facilitating N transport to TGN, the site of RNA replication.