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Sensitizer-Induced Basophils Accelerate Skin Re-Epithelialization via IL-4/IL-13-Mediated Macrophage Polarization - PubMed

5 days ago
  • #basophils
  • #IL-4/IL-13
  • #wound healing
  • Atopic dermatitis (AD) patients show rapid re-epithelialization after scratching despite impaired skin barriers.
  • Basophils, key in allergic inflammation, were found to contribute to wound healing in sensitized skin responses.
  • Single-cell RNA sequencing and flow cytometry showed basophil enrichment in wounded skin, peaking at day 3-6 post-injury.
  • Sensitizer-challenge enhanced basophil recruitment, accelerating wound closure, angiogenesis, and re-epithelialization.
  • Depletion of basophils impaired repair processes, highlighting their critical role.
  • Basophils were the main source of IL-4 and IL-13, driving macrophage polarization to a pro-repair M2 phenotype.
  • Loss of IL-4/IL-13 in basophils caused healing defects, rescued by local cytokine administration.
  • The study reveals a novel pro-repair function of basophils via IL-4/IL-13-dependent macrophage reprogramming.