Endocytome profiling uncovers cell-surface protein dynamics underlying neuronal connectivity - PubMed
4 hours ago
- #neuronal connectivity
- #Drosophila
- #proteomics
- Endocytome profiling is a systematic approach to map cell-surface protein (CSP) dynamics in situ.
- Quantitative proteomic analysis in Drosophila olfactory receptor neuron (ORN) axons identified over 1,000 proteins in an endocytic atlas.
- The study reveals how the cell-surface proteome is remodeled to meet developmental demands.
- A junctional CSP's endosome-to-surface ratio is critical for axon pruning while maintaining mature axon integrity.
- Multi-omic integration identified transcellular signaling and a growth factor secreted by neighboring neurons that directs ORN axon targeting.
- Endocytome profiling offers a platform to study proteome-scale remodeling across various cell types and contexts.