Mapping Cell Metabolic States by Image-Enabled Gating Metabolomic Cytometry - PubMed
4 hours ago
- #single-cell metabolomics
- #metabolic cytometry
- #cell atlases
- CyMeta-ImaGating is introduced for generating single-cell metabolic atlases with verified profiles.
- Image-enabled gating matches metabolomic profiles with brightfield single-cell images, improving single-cell profile accuracy from 28% to 91-100%.
- The method was applied to scraped HeLa cell suspensions, effectively distinguishing single cells from debris and aggregates.
- Spleen cell atlases generated using CyMeta-ImaGating distinguished major immune cell types (B, T, NK cells) and metabolic state transitions post-pathogen activation.
- Doxorubicin's effects were characterized using liver and cancer cell atlases, revealing specific metabolic states during apoptosis and drug response robustness in cancer cells.