Non-propagating RNA virus-vectored HA/NA vaccine prevents shedding of antigen-drifted H1N1 influenza virus in pigs - PubMed
8 hours ago
- #influenza vaccine
- #antigenic drift
- #porcine model
- A non-propagating vesicular stomatitis virus (VSV) vector encoding HA and NA antigens of H1N1 was tested in pigs to induce cross-reactive immunity against antigenic drift.
- A single intramuscular immunization elicited high titers of neutralizing antibodies and N1-sialidase inhibition, with a boost enhancing activity against the drifted H1N1 strain.
- The vaccine induced N1-specific antibodies that inhibited avian N1 sialidase and suppressed replication of bovine-derived H5N1 virus in vitro.
- After nasal challenge with a drifted reassortant virus, vaccinated pigs showed no detectable virus shedding, while control animals shed infectious virus.
- Homologous prime-boost vaccination with VSV-vectored antigens conferred protection comparable to a heterologous VSV/LAIV regimen in the upper respiratory tract.