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High-throughput ligand diversification to discover chemical inducers of proximity - PubMed

6 days ago
  • #protein degradation
  • #chemical inducers of proximity
  • #high-throughput chemistry
  • High-throughput ligand diversification used to discover chemical inducers of proximity (CIPs).
  • Sulfur(VI) fluoride exchange-based high-throughput chemistry (HTC) installed 3,163 building blocks on ENL and BRD4 ligands.
  • Identified dHTC1, an ENL degrader recruiting CRL4CRBN complex through an extended protein-protein interface.
  • Discovered dHTC3, a molecular glue selectively dimerizing BRD4 bromodomain 1 to SCFFBXO3.
  • HTC introduced as a tool for discovering new CIPs and effectors for proximity pharmacology.
  • Multiple authors have competing interests, including patents, equity, and advisory roles in biotech companies.
  • Research funded by various grants from NIH, NSF, and international organizations.