Integrative CSF profiling identifies disease-specific immune responses in leptomeningeal disease - PubMed
4 days ago
- #Liquid biopsy
- #Leptomeningeal disease
- #Immune profiling
- Leptomeningeal disease (LMD) is a severe condition in advanced cancer with rapid neurological decline and few treatment options.
- Single-cell RNA and T cell receptor (TCR) sequencing of cerebrospinal fluid (CSF) from patients with CNS lymphoma (CNSL), brain metastases (BrMs), and glioblastoma (GB) was conducted.
- Distinct, disease-specific immune responses were found: CNSL-associated LMD showed clonal T cell expansion, while BrMs and GB had more blood-derived and resident-like myeloid cells.
- Spatial transcriptomics confirmed similarities between CSF and tumor microenvironments.
- Longitudinal sampling revealed dynamic immune changes and emerging resistant clones.
- CSF is identified as an immune-active compartment reflecting disease-specific features, useful for immune monitoring and therapeutic stratification in LMD.