Methylglyoxal Affects Dopamine Homeostasis in SH-SY5Y Cells Through the Modulation of miR-190a and miR-214 - PubMed
4 days ago
- #microRNA
- #methylglyoxal
- #dopamine
- Methylglyoxal (MGO) affects dopamine (DA) homeostasis in SH-SY5Y cells.
- MGO reduces intracellular DA content and increases expression of proteins (COMT, MAO, α-Syn) that negatively affect DA levels.
- MGO significantly decreases the expression of miR-190a and miR-214.
- miR-190a and miR-214 are predicted regulators of COMT and SNCA (α-synuclein gene).
- Gain- and loss-of-function experiments show these miRNAs modulate COMT and SNCA expression.
- COMT is a direct target of both miR-190a and miR-214, while SNCA is a direct target of miR-190a and an indirect target of miR-214.
- Transfection of miR-190a and miR-214 mimics reverses MGO effects on COMT and α-Syn expression and restores DA levels.
- These miRNAs could be pharmacological targets for treating diabetes-associated dopaminergic dysfunction and cognitive decline.