A novel humanized immune stroma PDX cancer model for therapeutic studies - PubMed
6 hours ago
- #PDX Model
- #Humanized Stroma
- #Immunotherapy
- A new HS-PDX model was developed with patient-derived tumor cells, mesenchymal stem/progenitor cells, and human endothelial cells, incorporating a human tumor stroma to better mimic therapeutic resistance.
- HS-PDX shows increased resistance to targeted therapy and chemotherapy compared to standard PDX models, aligning more closely with patient responses.
- Extending HS-PDX to HIS-PDX by using mice with humanized bone marrow adds human immune cell infiltrates, enhancing the model's relevance for immunological studies.
- RNA sequencing reveals a 94-96% correlation between HIS-PDX and primary human tumors, validating its accuracy in representing human tumor biology.
- The model demonstrates the stroma's role in recruiting tumor-associated macrophages and promoting immune exclusion, highlighting its immunosuppressive effects and utility in testing immunotherapy combinations.