A Physiological Microfluidic Blood-Brain-Barrier Model for In Vitro Study of Nanoparticle Trafficking and Accumulation - PubMed
4 hours ago
- #Blood-Brain Barrier
- #Microfluidic Model
- #Nanoparticles
- Development of a microfluidic BBB model (mBBB) using human cerebral microvascular endothelial cells (HCMECs) with astrocytes and pericytes.
- The model enables high-resolution imaging and direct assessment of nanoparticle transport and accumulation.
- Recapitulates key BBB features like selective permeability, junctional protein expression, and receptor-mediated uptake.
- Comparison of nanoparticle trafficking shows heterologous extracellular vesicles (EVs) have the highest transport efficiency.
- Nanoparticle properties like ligand presentation and membrane composition primarily govern BBB penetration, not size or stiffness.
- The mBBB platform offers a high-throughput, imaging-based tool for drug delivery and neurotoxicity screening.