Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens - PubMed
2 days ago
- #genetic screens
- #Arabidopsis
- #CRISPR
- Development of multiplexed CRISPR libraries for Arabidopsis genetic screens.
- Each construct contains two sgRNAs targeting multiple gene family members.
- Enables cell-type-specific and tissue-level genetic screens.
- Double-barcoding strategy for efficient tracking of sgRNA combinations.
- Generated over 1,000 Arabidopsis lines targeting 707 transporter genes.
- Increases gene coverage and editing efficiency.
- Scalable resource for multi-targeted genome editing in plants.