Bioactivity-guided isolation of anti-inflammatory compounds from the herbs constituting a traditional Chinese medicine formulation used for the treatment of rheumatoid arthritis - PubMed
3 hours ago
- #Anti-inflammatory Compounds
- #Rheumatoid Arthritis
- #Traditional Chinese Medicine
- Bioactivity-guided isolation of anti-inflammatory compounds from a Traditional Chinese Medicine (TCM) formulation for rheumatoid arthritis (RA) treatment.
- RA is a chronic inflammatory autoimmune disease with significant socio-economic impact.
- TCM herbal formulations are promising sources for novel anti-rheumatic drugs but face challenges due to RA complexity and chemical diversity.
- Study aimed to identify anti-inflammatory constituents from individual plants in 'Formulation A' and characterize their effects on inflammatory pathways.
- A phenotypic screening strategy was used to bridge single-species studies and formulation-level decoction studies.
- Custom in vitro assays (NF-κB, NFAT, STAT3, STAT5) and cytokine production evaluations were conducted.
- Strongest anti-inflammatory activities were found in nonpolar extracts, highlighting the utility of organic solvent extraction.
- Two new compounds from Atractylodes lancea and 37 known compounds with anti-inflammatory activities were identified.
- Study provides mechanistic insights into anti-inflammatory effects and establishes a screening platform for new anti-rheumatic agents.