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Promoter Hypermethylation-Induced Silencing of FXYD1 Drives Breast Cancer Metastasis via DDX5-Mediated Wnt/β-Catenin Pathway Activation - PubMed

3 days ago
  • #Epigenetics
  • #Wnt Signaling
  • #Breast Cancer
  • FXYD1 is identified as a significantly downregulated gene in breast cancer tissues, associated with poor patient prognosis.
  • Promoter hypermethylation drives the silencing of FXYD1 in breast cancer.
  • Restoration of FXYD1 suppresses cell proliferation, migration, and lung metastasis in vitro and in vivo.
  • FXYD1 recruits the E3 ubiquitin ligase MAEA to DDX5, promoting its degradation via K63-linked ubiquitination.
  • This process reduces β-catenin stability, impairs its nuclear translocation, and attenuates Wnt target gene expression.
  • The study uncovers the FXYD1-MAEA-DDX5 axis as a novel inhibitor of Wnt/β-catenin signaling.
  • FXYD1 is proposed as a potential tumor suppressor, prognostic biomarker, and therapeutic target in breast cancer.