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Atractylodis Rhizoma-Atractylodis Macrocephala Rhizoma herbal pair restores intestinal mucosal barrier function in ulcerative colitis via activating Epac1/Rap1 pathway and inhibiting PI3K/AKT pathway

8 hours ago
  • #intestinal barrier
  • #ulcerative colitis
  • #herbal medicine
  • Atractylodis Rhizoma-Atractylodis Macrocephala Rhizoma (AR-AM) herbal pair helps restore intestinal mucosal barrier function in ulcerative colitis (UC).
  • The study used a murine UC model induced by dextran sodium sulfate (DSS) to evaluate the therapeutic effects of AR-AM ethanolic extract (EEAR-AM).
  • EEAR-AM significantly improved symptoms like weight loss, colon shortening, and disease activity index (DAI) in UC mice.
  • It restored colonic tissue structure, reduced inflammation, and enhanced mucin MUC2 and tight junction protein expression.
  • Network pharmacology suggested EEAR-AM modulates the Epac1/Rap1 and PI3K/AKT pathways to improve intestinal barrier function.
  • Western blotting confirmed EEAR-AM activates Epac1/Rap1 while inhibiting PI3K/AKT in both UC mice and Caco-2 cells.