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Reconstitution of the uterine immune milieu after uterus or hematopoietic stem cell transplantation - PubMed

a day ago
  • #immune reconstitution
  • #hematopoietic stem cell transplantation
  • #uterus transplantation
  • Study investigates reconstitution of the human uterine immune system post uterus or hematopoietic stem cell transplantation (HSCT).
  • Single-cell RNA sequencing and flow cytometry used to determine donor vs. recipient cell origin in the endometrium.
  • Endometrial immune cell reconstitution observed after both uterus transplant and HSCT at transcriptomic, phenotypic, and spatial levels.
  • Recipient-derived immune cells replaced donor cells post uterus transplant; HSCT led to near-complete donor-derived immune reconstitution.
  • Tacrolimus-induced calcineurin-mediated inhibition of NFAT pathway did not prevent immune reconstitution, including tissue-resident lymphocytes.
  • One case showed immune cells of male origin reconstituting the endometrium post HSCT.
  • Findings provide insights into tissue immune system persistence and reconstitution in the uterus, an organ with continuous regeneration.