Terminal glucose as a receptor for adeno-associated virus 44.9 - PubMed
4 hours ago
- #gene therapy
- #glycan receptor
- #AAV44.9
- AAV44.9 is a novel adeno-associated virus (AAV) serotype with unique cell tropism and gene transfer efficiency.
- Originally discovered as a contaminant in SV15 adenovirus stock, AAV44.9 is phylogenetically related to AAVrh.8R.
- AAV44.9 shows promise for targeting photoreceptors, salivary glands, and other tissues.
- Glycan arrays and competition experiments identified terminal glucose and glucosamine on N-linked carbohydrates as key receptors for AAV44.9.
- Mutagenesis studies pinpointed specific amino acids responsible for glycan recognition and binding specificity.
- The study highlights the importance of understanding AAV44.9's natural tropism for gene therapy applications.
- Terminal glucose binding is critical for AAV44.9 transduction, distinguishing it from closely related AAVrh.8R.