CRISPR-based functional genomics for dissecting therapeutic dependency in primary acute myeloid leukemia samples - PubMed
3 hours ago
- #functional genomics
- #AML
- #CRISPR
- CRISPR-based functional genomics is used to study therapeutic dependencies in primary acute myeloid leukemia (AML) samples.
- The study developed an optimized CRISPR platform for functional genomics in patient-derived xenograft and primary AML samples.
- CRISPR-Cas9 knockout and CRISPR interference (CRISPRi) dropout screens identified essential cis-regulatory elements for leukemic growth.
- Pooled CRISPR perturbations combined with single-cell RNA sequencing (Perturb-seq) revealed changes in regulatory networks and cellular hierarchies in primary AML samples.
- The research provides a framework for using CRISPR-based functional genomics to study cancer dependencies and cellular heterogeneity in primary AML samples.