Intra-Tissue Bacteriome and Cellular Profiles in Periodontal Granulation Tissue From Osseous Defects and Extraction Sockets - PubMed
4 hours ago
- #mesenchymal stem cells
- #bacteriome
- #periodontitis
- Study investigates intra-tissue bacteriome and cell profiles in periodontal granulation tissue (PGT) from osseous defects and extraction sockets.
- PGT samples collected from 49 patients with severe periodontitis, including osseous defects (GT), extraction sockets (ST), and extracted root surfaces (RT).
- Control samples were excised pocket wall (PT) from surgical sites.
- GT and PT showed periodontal health-associated, commensal-enriched bacteriome profiles.
- RT and ST exhibited worse local periodontal conditions, enriched with periodontopathogens but depleted commensals.
- GT contained higher proportion of mesenchymal stem cells (MSCs) and fibroblasts, fewer natural killer cells compared to PT and ST.
- Pseudotime trajectory analysis revealed endothelial and epithelial differentiation fates from mesenchymal progenitors across tissues.
- GT showed less inflammatory infiltration and immunoreactivity of CD4+ and NKG2D+ than PT and ST.
- Findings suggest GT has potential periodontal health-associated features regarding clinical, bacteriome, and cellular attributes.
- Future translational studies recommended to explore GT as an alternative source of MSCs for periodontal regeneration.