Bioinformatics analysis and qRT-PCR validation of iron metabolism-related genes in pediatric asthma - PubMed
4 hours ago
- #Pediatric Asthma
- #Bioinformatics Analysis
- #Iron Metabolism
- Integrated three GEO datasets (GSE27011, GSE40888, GSE40732) with 283 pediatric asthma (PA) and control samples to analyze iron metabolism-related genes.
- Identified 15 iron metabolism-related differentially expressed genes (IMRDEGs) like C19orf12, IREB2, XK, GDF15 involved in energy metabolism and iron homeostasis.
- Developed a machine learning model with four key genes showing moderate diagnostic discrimination (AUC = 0.69).
- qRT-PCR validation confirmed C19orf12 upregulation and IREB2 downregulation in PA patients, aligning with bioinformatics findings.
- Immune infiltration analysis indicated differences in memory CD4 + T cells and mast cells, linking iron metabolism to immune mechanisms in asthma.