Mechanism of 30S subunit recognition and modification by the conserved bacterial ribosomal RNA methyltransferase RsmI - PubMed
3 hours ago
- #ribosome
- #RNA modification
- #methyltransferase
- Ribosomal RNA (rRNA) modifications, like the 2'-O-methylation of C1402 in 16S rRNA, are crucial for ribosome function and antibiotic susceptibility.
- The conserved bacterial methyltransferase RsmI modifies C1402, a buried nucleotide in the 30S ribosomal subunit.
- A 2.42 Å cryo-EM structure reveals RsmI anchors to the 30S subunit via conserved 16S rRNA interactions, reorganizing h44 for C1402 access.
- The RsmI C-terminal domain is essential for 30S subunit interaction, and RsmI activity depends on a divalent metal ion, suggesting a new class of RNA O-methyltransferases.
- RsmI's recognition of distant ribosome features and rRNA reorganization hints at a role in accurate 30S subunit biogenesis.