Oocyte vitrification disrupts zygotic genome activation in embryos by impairing maternal spliceosome translation and Crxos splicing - PubMed
4 hours ago
- #Alternative Splicing
- #Zygotic Genome Activation
- #Oocyte Vitrification
- Oocyte vitrification disrupts maternal mRNA translation in mouse oocytes, particularly suppressing spliceosome component genes like Phf5a.
- This leads to widespread alternative splicing defects in 2-cell embryos, especially reducing functional full-length Crxos (Egam1) transcript and increasing a non-functional truncated variant.
- Loss of functional Crxos impairs zygotic genome activation (ZGA) by affecting RNA Pol II recruitment and elongation, compromising embryonic development and global transcription.
- The study outlines a cascade from vitrification to developmental issues via translational suppression, spliceosome impairment, aberrant splicing, and ZGA disruption.