Ectopic expression of cytosolic DHODH uncouples de novo pyrimidine biosynthesis from mitochondrial electron transport - PubMed
4 days ago
- #genetic tool
- #pyrimidine biosynthesis
- #mitochondrial electron transport
- Ectopic expression of cytosolic DHODH (ScURA) in mammalian cells uncouples pyrimidine biosynthesis from mitochondrial electron transport.
- ScURA uses fumarate as an electron acceptor instead of ubiquinone, enabling respiration-independent pyrimidine biosynthesis.
- Cells expressing ScURA are resistant to drugs inhibiting complex III and mitochondrial ribosome.
- ScURA supports growth of mitochondrial-DNA-lacking cells in uridine-deficient medium and ameliorates mitochondrial disease phenotypes.
- This genetic tool highlights the role of pyrimidine biosynthesis in electron transport chain defects.