Single-cell and isoform-specific translational profiling of the mouse brain - PubMed
4 days ago
- #translational profiling
- #mouse brain
- #single-cell RNA sequencing
- Single-cell and isoform-specific translational profiling of the mouse brain was conducted using Ribo-STAMP coupled with single-cell RNA sequencing.
- The study generated the first isoform-sensitive single-cell translatomes of the mouse hippocampus at postnatal day 25.
- Discovered cell-type-specific translation of 3,857 alternative transcripts across 1,641 genes.
- Identified isoforms of the same genes undergoing differential translation within and across 8 different cell types.
- Defined high and low translational states in CA1 and CA3 neurons, with synaptic and metabolic genes enriched in high states.
- CA3 neurons exhibited higher basal translation compared with CA1, confirmed by metabolic labeling and immunohistochemistry.
- The platform aims to expand understanding of how cell-type-specific and isoform-specific translation drives brain physiology and disease.
- Conflict of interest noted for G.W.Y. with Eclipse BioInnovations; other authors declared no competing interests.