An activated wheat CCG10-NLR immune receptor forms an octameric resistosome - PubMed
3 hours ago
- #octameric resistosome
- #plant immunity
- #CCG10-NLRs
- An activated wheat CCG10-NLR immune receptor forms an octameric resistosome.
- A gain-of-function mutant of wheat autoimmunity 3 (WAI3GOF) was identified, encoding a constitutively active CCG10-NLR due to a residue substitution in the LRR domain.
- Cryo-EM analysis shows that activated WAI3 assembles into a distinctive octameric resistosome.
- Arabidopsis RPS2, another CCG10-NLR, also forms an octamer, indicating a conserved structural property across monocot and dicot plants.
- The WAI3 resistosome induces a prolonged and sustained increase in cytosolic calcium, likely facilitated by a unique channel architecture from its divergent CC domain configuration.
- This domain arrangement may be shared by plant NLRs lacking the conserved EDVID motif in their CC domains.
- The findings uncover a conserved yet previously uncharacterized NLR resistosome structure and provide insights into plant immune receptor plasticity.