Novel Midkine-Derived Peptide Promotes Reparative Dentine Formation via DDIT4/mTOR Pathway-Mediated Activation of Autophagy - PubMed
6 hours ago
- #Dentine Regeneration
- #Peptide Therapy
- #Autophagy
- Midkine (MK) protein promotes reparative dentine formation, but direct recombinant protein use has clinical and cost limitations.
- A novel MK-derived peptide, MK-12 (sequence KARYNAQCQETI), was developed and showed highest mineralization potential among 15 peptides.
- MK-12 demonstrated in vitro biocompatibility below 200 μg/mL, with optimal effects on cell migration and odontogenic differentiation at 100 μg/mL.
- MK-12 promotes reparative dentine formation by upregulating DDIT4, which modulates the mTOR pathway to activate autophagy.
- In vivo rat pulp capping model confirmed MK-12 effectively induces reparative dentine formation with good biocompatibility.