Patient-derived ependymoma and medulloblastoma tumoroids: generation, biobanking and drug screening - PubMed
8 hours ago
- #pediatric brain tumors
- #tumoroids
- #drug screening
- Protocol for generating pediatric patient-derived tumoroids (pPDTs) and xenograft tumoroids (pPDXTs) from ependymoma and medulloblastoma tumors.
- Expands on previous methods for cancer organoids, sharing reagents and steps for efficient tumoroid setup, amplification, biobanking, and cryopreservation.
- Includes a scalable drug screening approach using calcein-based staining and automated image analysis to assess therapeutic responses quickly.
- Aims to provide a reproducible in vitro model for studying pediatric brain tumors, supporting mechanistic and preclinical research in neuro-oncology.