Viperin inhibits coronavirus replication by targeting non-structural protein 8 to disrupt replication-transcription complex assembly and RNA-dependent RNA polymerase activity - PubMed
6 hours ago
- #Coronavirus
- #Antiviral Mechanism
- #Viperin
- Viperin, an interferon-stimulated gene product, broadly inhibits viruses by converting CTP to ddhCTP via its SAM enzymatic activity, causing RNA synthesis termination.
- This study uncovers a novel anti-coronavirus mechanism where viperin targets non-structural protein 8 (nsp8) to disrupt replication-transcription complex assembly and reduce RdRp activity.
- Using Porcine Deltacoronavirus as a model, viperin was induced upon infection and inhibited replication, with the central domain of viperin and lysine 82 in nsp8 being critical for interaction.
- The viperin-nsp8 interaction is conserved across all coronavirus genera (α, β, γ, δ), highlighting its potential as a therapeutic target against nsp8 function.