Ex vivo and in vivo CRISPR/Cas9 screenings identify the roles of protein N-glycosylation in regulating T-cell activation and functions - PubMed
3 hours ago
- #T-cells
- #Immunotherapy
- #CRISPR
- CRISPR/Cas9 screenings identify roles of protein N-glycosylation in T-cell activation and functions.
- Inactivation of B4GALT1 enhances TCR activation and CD8+ T-cell functions but does not affect CAR-T cells.
- B4GALT1 galactosylates TCR and CD8, reducing their interaction essential for TCR activation.
- A TCR-CD8 fusion protein can bypass B4GALT1 regulation in CD8+ T-cells.
- B4GALT1 expression levels in tumor-infiltrated CD8+ T-cells negatively correlate with patient prognosis.
- B4GALT1 is a potential target for tumor immunotherapy.