Supercoiled DNA recognition and cleavage control in topoisomerase VI - PubMed
5 days ago
- #supercoiled DNA
- #enzyme mechanism
- #DNA topoisomerase
- Type IIB topoisomerases, including DNA topoisomerase VI (Top6), are crucial for DNA supercoiling modulation and chromosome disentanglement in plants, archaea, and eukaryotes.
- Top6 is involved in endoreduplication, cell proliferation, meiotic recombination initiation, and bacterial defense systems like MksBEFG/Wadjet/Gabija.
- Structures of Top6 bound to supercoiled minicircles reveal it binds a curved 74 bp DNA region and cleaves at a deformability motif, explaining its preference for supercoiled DNA.
- Dynamic protein-DNA interactions and a tension sensor help recognize bent DNA and link ATPase disposition to cleavage activation.
- The study highlights how DNA plasticity and structural changes regulate DNA recognition and cleavage by type II topoisomerases.
- Authors J.M.F. and L.Z. hold patents on minicircle technology and are shareholders in Twister Biotech, Inc. (Velvet Therapeutics, Inc.).