Cryopreservative Bioink Enables Direct Bioprinting of Adherent Cells - PubMed
a day ago
- #3D bioprinting
- #tissue regeneration
- #bioink cryopreservation
- Cryopreservation-integrated bioprinting combines cell-laden bioink freezing with direct post-thaw printing, bypassing traditional culturing steps.
- Key challenges include ice crystallization compromising cellular viability and hydrogel structural integrity deterioration impairing printability.
- CAMP (Cryopreservation for Adhesion and Maintenance Printing) is a biphasic bioink platform enabling direct 3D printing at 4-8°C post liquid nitrogen storage (-196°C).
- CAMP inhibits ice recrystallization through hydrogen bond-mediated water immobilization, achieving ~80% cell viability without toxic cryoprotectants.
- Cryopreserved cells in CAMP retain focal adhesions and show increased phosphorylated FAK expression.
- CAMP exhibits ~10x higher ice recrystallization inhibition than phosphate-buffered saline.
- Mechanistically, CAMP suppresses cell death via phospho-FAK signaling.
- In vivo evaluation using a rat femoral defect model showed CAMP cryopreserved constructs promote complete bone regeneration within three months.
- CAMP combines cell cryopreservation, bioprinting, and functional tissue formation into a single workflow, overcoming conventional biofabrication limitations.
- CAMP represents a significant advance toward clinically viable, ready-to-implant engineered tissues.