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ASCT2 palmitoylation regulated by JNK1-ZDHHC14 axis orchestrates glutamine metabolism and NSCLC progression - PubMed

3 hours ago
  • #NSCLC
  • #ASCT2
  • #palmitoylation
  • ASCT2 palmitoylation is regulated by the JNK1-ZDHHC14 axis, impacting glutamine metabolism and NSCLC progression.
  • ZDHHC14 is identified as the key palmitoyltransferase for ASCT2 at Cys39 and Cys48, promoting its lysosomal degradation, while ABHD17B acts as a depalmitoylase to stabilize ASCT2.
  • Glutamine deprivation activates JNK1, which phosphorylates ZDHHC14 at Thr440, leading to its degradation and increased ASCT2 stability.
  • Combining JNK and ASCT2 inhibitors synergistically inhibits glutamine metabolism and tumor growth in vivo.
  • The study reveals a phosphorylation-palmitoylation axis connecting JNK-mediated ASCT2 palmitoylation to glutamine metabolism, suggesting a therapeutic strategy for NSCLC.