Comprehensive Analysis of the Expression and Prognostic Significance of NPAS1 in Patients with Colorectal Adenocarcinoma Based on Bioinformatics - PubMed
8 hours ago
- #NPAS1
- #colorectal adenocarcinoma
- #bioinformatics
- NPAS1 is a protein-coding gene primarily expressed in the central nervous system and plays a role in nervous system development.
- The study analyzed NPAS1 expression in colorectal adenocarcinoma (COAD) using TCGA and GEO data, validated by Western blotting and immunohistochemical staining.
- Higher NPAS1 expression in normal tissues compared to COAD tissues was associated with better survival outcomes.
- Bioinformatics analysis linked NPAS1 co-expressed genes to diverse signaling pathways and cellular functions.
- NPAS1 expression correlated with certain immune cells (CD56bright, cytotoxic, NK cells) and negatively correlated with helper T cells and Tcm cells.
- DNA methylation levels of NPAS1 were higher in tumor tissues compared to normal tissues.
- The study explored NPAS1 mutation characteristics, single-cell expression profiling, and protein-protein interactions.
- Findings provide insights into the etiology, identification, and management of COAD.
- NPAS1 expression in COAD is linked to clinical stage, prognosis, immune infiltration, and DNA methylation.