A roadmap for systematic humanization of a Chimeric Antigen Receptor: pre-clinical validation of a humanized CD22 scFv as a model - PubMed

4 hours ago

Humanization of CAR T-cell constructs is vital to lower immunogenicity and maintain antigen-binding affinity.
A structure-guided method was used for CD22-targeted CAR-T cells, grafting murine CDRs onto human frameworks.
In silico and biophysical screening identified and fixed issues like abnormal glycosylation sites and instability.
The humanized CD22-CAR constructs kept antigen recognition, specificity, and cytotoxic power similar to murine versions.
Tests in lab settings and animal models confirmed the approach's effectiveness for reducing immunogenicity while preserving therapy efficacy.

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