Purification of the Active-State G Protein-Coupled Receptor ADGRL4 for Cryo-Electron Microscopy Using a Modular Tag System and a Tethered mini-Gq - PubMed
7 hours ago
- #cryo-EM
- #GPCR purification
- #ADGRL4
- Purification of active-state ADGRL4 for cryo-EM using a modular tag system and tethered mini-Gq.
- ADGRL4 is an adhesion GPCR involved in tumor progression.
- Workflow includes a multifunctional tagging strategy for purification and detection.
- Tethered mini-Gq enhances receptor stability and cell-surface expression.
- Method is adaptable to other weakly coupling GPCRs.
- Open access article under CC BY-NC license.