High-throughput sperm screening using one-step RT-qPCR: Improvement and re-evaluation - PubMed
4 hours ago
- #Sperm Detection
- #Forensic Science
- #RT-qPCR
- High-throughput sperm screening using one-step RT-qPCR improves efficiency in sexual assault cases.
- The study targets PRM2 mRNA for sperm detection, achieving high specificity and sensitivity (detects as low as 0.01 μL of semen).
- The assay simplifies workflow: RNA extraction takes <30 min and RT-qPCR <60 min.
- Successful detection of PRM2 mRNA in simulated-case samples demonstrates practical applicability.
- Offers a promising strategy for high-throughput sperm screening in forensic workflows.