Imaging and genetic toolbox to study Arabidopsis embryogenesis - PubMed
a day ago
- #embryogenesis
- #Arabidopsis
- #auxin
- Arabidopsis thaliana embryogenesis provides a model for understanding plant body plan establishment.
- Asymmetric zygote division establishes the apical-basal axis, defining future shoot and root poles.
- Key genetic mutants (gnom, monopteros, bodenlos, topless) highlight auxin's role in axis formation and organ initiation.
- Polarized PIN auxin transporters and transcription factors (MONOPTEROS, WUSCHEL-RELATED HOMEOBOX) regulate development.
- Advanced microscopy techniques enable real-time observation and quantitative analysis of embryo development.
- Fluorescence-compatible clearing reagents and 3D reconstructions allow study of cell geometry and division orientation.
- Live ovule imaging and laser-assisted cell ablation probe cell communication and fate plasticity.
- Arabidopsis embryos are a prime model for studying chemical and biophysical developmental cues.