Co-opting the bacterial lipoprotein pathway for the biosynthesis of lipidated macrocyclic peptides - PubMed
4 hours ago
- #Macrocyclic Peptides
- #RiPPs
- #Bacterial Lipoprotein Pathway
- The study co-opts the bacterial lipoprotein pathway to synthesize lipidated macrocyclic peptides.
- It focuses on RiPPs (ribosomally synthesized and posttranslationally modified peptides), which have diverse bioactivities.
- Enzymes like ChrH catalyze complex modifications, creating structures like macrocycles and heterocycles.
- Comparative genomics reveals structural diversity, including unmethylated macrocyclic forms and large C-terminally modified proteins.
- Some precursors have signal peptides, directing them to the bacterial lipoprotein pathway for maturation.
- This process adds a diacylglycerol (DAG) group to the N-terminal cysteine and acylates the amine.
- These hybrids, termed DAG-RiPPs, are widespread across bacteria and may serve various biological roles.
- The findings highlight a new maturation paradigm for membrane-bound RiPPs and support future bioengineering.