SLC29A1/ENT1 and SLC29A3/ENT3 differentially regulate autophagy - PubMed
4 hours ago
- #nucleoside transporters
- #AMPK
- #autophagy
- SLC29A1/ENT1 and SLC29A3/ENT3 have distinct roles in regulating autophagy.
- SLC29A1/ENT1 inhibits autophagy by suppressing PRKAA/AMPK phosphorylation, while SLC29A3/ENT3 promotes autophagy.
- Adenosine (Ado) triggers PRKAA/AMPK phosphorylation and autophagy in a concentration-dependent manner.
- SLC29A1 promotes Ado efflux, reducing intracellular Ado and attenuating autophagy.
- Pharmacological inhibition of SLC29A1 enhances intracellular Ado accumulation and autophagy.
- SLC29A1 promotes BECN1-BCL2 interaction, inhibiting autophagosome formation, unlike SLC29A3 which modulates the MTOR pathway.
- Autophagy differentially regulates SLC29A1 and SLC29A3 expression, with compensatory upregulation observed.
- slc29a1-/- and slc29a3-/- mice show autophagic proficiency and deficiency, respectively.