Mycobacterium tuberculosis MutT4/RppH is an RNA pyrophosphohydrolase that forms condensate-like bodies and impacts mRNA degradation - PubMed
7 hours ago
- #Mycobacterium tuberculosis
- #Biomolecular condensates
- #RNA degradation
- Mycobacterium tuberculosis MutT4/RppH is identified as an RNA pyrophosphohydrolase.
- It converts mRNA 5'-triphosphates to 5'-monophosphates, initiating mRNA degradation.
- Deletion of rppHMtb increases 5'-triphosphates on mRNAs but has mixed effects on degradation in vivo.
- RppHMtb forms condensate-like bodies with RNases, dissociating upon rifampicin addition.
- The N-terminal intrinsically disordered region (IDR) of RppHMtb is sufficient for condensate formation.
- Deletion of rppHMtb leads to higher outer membrane permeability and oxidative stress resistance.