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Carotid plaque macrophage burden and inflammatory lipid-associated macrophage markers predict secondary major adverse cardiovascular events after endarterectomy - PubMed

3 hours ago
  • #Atherosclerosis
  • #Macrophages
  • #Cardiovascular Risk
  • Atherosclerosis is a chronic lipid-driven inflammatory disease and a leading cause of cardiovascular morbidity and mortality.
  • Macrophages play a key role in atherosclerotic development, but their subpopulations lack detailed characterization.
  • Single-cell RNA sequencing was performed on blood and plaques from 46 carotid endarterectomy patients.
  • Four major types of plaque macrophages were identified: inflammatory macrophages, lipid-associated macrophages (LAMs), tissue-resident-like LAMs, and inflammatory LAMs.
  • Monocytes differentiate into inflammatory LAMs via various stages, contributing to plaque progression.
  • Macrophages were the only cell population significantly associated with symptoms at surgery and increased risk of major adverse cardiovascular events within 3 years.
  • LAM and inflammatory LAM markers (PLIN2, TREM1) were linked to higher cardiovascular risk post-surgery.
  • Findings were validated in an independent cohort (Carotid Plaque Imaging Project).
  • The study provides insights into macrophage subpopulations and their clinical significance in predicting cardiovascular events.