Single-Cell Analysis Identified a Recurrent Malignant-Associated Transcriptional State in Colorectal Cancer - PubMed
4 hours ago
- #colorectal cancer
- #transcriptional state
- #single-cell RNA sequencing
- Single-cell RNA sequencing (scRNA-seq) was performed on tumor and adjacent normal tissues from four Japanese colorectal cancer (CRC) patients.
- A common cancer-associated cell cluster was identified across all cases, characterized by high expression of the long noncoding RNA KCNQ1OT1.
- RASSF6 expression was markedly decreased in this cluster, contrasting with Western cohorts where elevated RASSF6 correlates with poor survival.
- Reanalysis of bulk RNA-seq data from East Asian cohorts confirmed reduced RASSF6 in tumors, while KCNQ1OT1 showed no significant differences.
- The study highlights the value of single-cell profiling in uncovering distinct gene expression features in CRC that bulk analyses may miss.