Structure of the mouse cytoplasmic lattice - PubMed
7 hours ago
- #cytoplasmic lattice
- #maternal regulation
- #cryo-EM structure
- The cryo-EM structure of a native mouse cytoplasmic lattice (CPL) repeating unit (~4 MDa) at 3.74 Å resolution reveals a tripartite architecture: framework, extended linkers, and core.
- The external framework is built from PADI6 decamers and subcortical maternal complexes (SCMC), linked into filaments by NLRP4F polymers.
- The CPL core traps epigenetic regulator UHRF1 in an autoinhibited conformation via PADI6, UBE2D, and NLRP14, preventing nuclear entry and ubiquitin ligase activity.
- CPL core stores GTP-bound α/β-tubulin heterodimers and inactive SCF E3-ubiquitin ligase components (FBXW-SKP1 complex) in a poised but restrained state.
- CPLs function as dynamic regulatory pools for rapid microtubule assembly and controlled ubiquitination during the oocyte-to-embryo transition, acting as specialized proteostasis organelles.