Deep homology and design of proteasome chaperone proteins in Candidozyma auris - PubMed
8 hours ago
- #structural homology
- #protein design
- #proteasome chaperones
- Protein structure mediates the sequence-function relationship, and structural similarity can identify rapidly evolving proteasome assembly chaperones in Candida auris.
- Function is conserved despite sequence divergence, indicating that specific folds, not sequences, are required for function, allowing structurally similar proteins to be functionally interchangeable even without common evolutionary history.
- Structure-informed protein design enabled mutation of over 40 residues in the Poc4 chaperone, demonstrating that artificial proteins can rescue complex biological processes in whole cells, expanding the ability to use structure for evolutionary and functional predictions.