RF-SIRF reveals a replication stress-specific epigenetic code by spatio-temporal mapping of reversed forks - PubMed
4 hours ago
- #genomic stability
- #epigenetics
- #DNA replication
- RF-SIRF is a new method for mapping reversed forks (RFs) with single-cell resolution.
- RFs accumulate at the nuclear periphery during early-mid S-phase.
- RFs have a specialized chromatin landscape and an epigenetic replication stress code distinct from transcription.
- RF-SIRF enables quantitative, temporal, spatial, and proteomic analyses of RFs.
- The method empowers advanced cellular and medical investigations of DNA replication stress responses.