ATP1A1 enhances porcine reproductive and respiratory syndrome virus type 2 attachment and internalization - PubMed
4 hours ago
- #PRRSV-2
- #virus entry
- #ATP1A1
- ATP1A1 enhances PRRSV-2 attachment and internalization.
- ATP1A1 clusters in the plasma membrane early in PRRSV-2 infection and co-internalizes with virions.
- Knockdown of ATP1A1 suppresses PRRSV-2 infection by reducing viral attachment.
- Chemical ligands ouabain and PST2238 reduce viral internalization without affecting attachment.
- ATP1A1-Src signaling activates EGFR and caveolin-1 for PRRSV-2 uptake via macropinocytosis and caveolae/raft-mediated endocytosis.
- PRRSV glycoprotein GP4 interacts with ATP1A1's fourth extracellular region (ER4).
- A synthetic ATP1A1-ER4 peptide inhibits PRRSV-2 replication by competitively reducing attachment and internalization.
- A nanobody targeting ATP1A1-ER4 broadly inhibits various PRRSV-2 lineages.
- Findings offer potential targets for antiviral treatments against PRRSV-2.