Mechanism of beta-arrestin 1 mediated Src activation via Src SH3 domain revealed by cryo-electron microscopy - PubMed
a day ago
- #Cryo-EM
- #Beta-arrestin
- #Src activation
- Beta-arrestin 1 (βarr1) mediates Src activation via interactions with the Src SH3 domain.
- Two distinct binding sites on βarr1 engage the Src SH3 domain: a polyproline motif in the N-domain and a non-proline-based interaction in the central crest region.
- These interactions disrupt Src's autoinhibited conformation, leading to allosteric activation.
- The study positions βarr1 as an active regulatory protein rather than a passive scaffold in signaling pathways.
- Findings suggest a general mechanism for βarr-mediated signaling across diverse effectors.