Depolymerizing F-actin accelerates the exit from pluripotency to enhance stem cell-derived islet differentiation - PubMed
4 hours ago
- #stem cell differentiation
- #F-actin depolymerization
- #pancreatic islets
- Depolymerizing F-actin with latrunculin A (latA) during the first 24 hours of definitive endoderm formation enhances the exit of human pluripotent stem cells (hPSCs) from pluripotency.
- The treatment alters key signaling pathways (Activin/Nodal, BMP, c-Jun, WNT), leading to improved pancreatic progenitor identity and reduced off-target lineage markers.
- This results in islets with more β cells, better maturation, insulin secretion, and ability to reverse hyperglycemia in disease models.
- The approach also reduces enterochromaffin cells and improves differentiation consistency across hPSC lines that previously struggled to produce pancreatic islets.