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A Visual Guide to DNA Sequencing

6 hours ago
  • #biotechnology
  • #genomics
  • #DNA sequencing
  • The Human Genome Project (HGP) released its initial draft sequence in 2001, hailed as a groundbreaking achievement.
  • Sanger sequencing, developed by Fred Sanger, was a pivotal method in DNA sequencing, earning him two Nobel Prizes.
  • Pyrosequencing, developed by Pål Nyren, introduced a new sequencing chemistry that was automated and gel-free.
  • Illumina's sequencing by synthesis method became the most common next-generation sequencing (NGS) technology, known for its accuracy and versatility.
  • PacBio's SMRT sequencing offers long reads, useful for complex genomes with repetitive sequences.
  • Nanopore sequencing, the most recent major method, allows for real-time, long-read sequencing with portable devices like the MinION.
  • DNA sequencing costs have plummeted over the years, from $3 billion for the HGP to under $100 per genome in recent advancements.
  • Future sequencing technologies aim to further reduce costs and improve accuracy, with ongoing innovations like Sequencing by Expansion.