Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activation - PubMed
4 days ago
- #loop extrusion
- #NIPBL
- #transcription regulation
- Acute depletion of NIPBL reveals in vivo dynamics of loop extrusion and its role in transcription activation.
- Many chromatin loops diminish rapidly upon NIPBL loss, but some persist for hours, requiring NIPBL for establishment during mitotic exit.
- Persistent loops are associated with distinct chromatin states and depend on STAG1 for their persistence.
- NIPBL depletion in multiple cell types shows its specific regulation of cell identity genes by supporting unique local genome conformations.
- NIPBL-mediated loop extrusion is critical for genome organization and transcription regulation in vivo.